Sitedirected mutagenesis

>> YOUR LINK HERE: ___ http://youtube.com/watch?v=00ASbLdKEhg

Site-directed mutagenesis is a laboratory/PCR based technique used to introduce specific mutations into a DNA sequence. This method allows researchers to study the effects of mutations on the function of a gene or protein, and can be used to create new variants with desired properties. Site-directed mutagenesis can be performed using PCR-based or cloning-based methods, and has many applications in both basic research and biotechnology, including studying protein function and engineering enzymes with improved activity or specificity. • There are various techniques involved are: • Quick change method • Overlap Primer based method • inverse PCR based • Megaprime based method • Here in this lecture we will discuss on Quick change method and Overlap Primer based method. • #SiteDirectedMutagenesis • #SDM • #Mutagenesis • #ProteinEngineering • #GeneticEngineering • #MolecularBiology • #PCR • #Cloning • #EnzymeEngineering • #GenomeEditing • #biotechnology • #genetics • #csir • SDM lecture 2 : • Site Directed Mutagenesis : Inverse a...

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