Gene Therapy and Sickle Cell Disease

>> YOUR LINK HERE: ___ http://youtube.com/watch?v=1BSiFfyObTY

Due to CRISPR's unparalleled ease-of-use and affordability, gene knockout experiments are now more feasible than ever before! • In this video, we will show you how we used the CRISPR Cas9 system to knockout the LIF gene in a colon carcinoma cell line. This is accomplished by the delivery of a Cas9 and sgRNA lentivector into the cell line followed by drug selection and monoclonal isolation. More importantly, we demonstrate how to characterize and verify your CRISPR Knockout using the PCR-based Surveyor Assay, Sanger Sequencing, and Next Generation Sequencing. This complete process of validation ensures that your final CRISPR Knockout cell line is 100% successful. • At the end of the video, we will introduce you to abm’s entire suite of CRISPR Knockout services – designed to help you jumpstart your Knockout experiments! • Read our complete CRISPR Knockout Case Study here: • ➜ https://info.abmgood.com/crispr-cas9-... • Want more tips, tricks, guides, and protocols on how to knockout a gene using CRISPR? Join our free, 4-week #CRISPRCrashCourse: http://info.abmgood.com/crispr-crash-... • Read more about the basics of the CRISPR system on our knowledge base: • ➜ https://info.abmgood.com/crispr-cas9 • Learn more about our CRISPR Knockout Cell Line Generation Service here: • ➜ https://goo.gl/EpEZDB • Watch more videos about CRISPR: • ➜CRISPR Cas9 - Gene Regulation with dCas9: https://goo.gl/tRkHWd • ➜ How a mini CRISPR Cas9 system is revolutionizing gene therapy! https://goo.gl/njRPv2 • Thank you to all of the amazing community contributions towards translating this video into other languages including: • ➜ Spanish • (To view the translations, toggle the CC button and then go to Settings ➜ Subtitles/CC to select a language.) • Connect with us on our social media pages to stay up to date with the latest scientific discoveries: • ➜ Facebook: https://goo.gl/hc9KrG • ➜ Twitter: https://goo.gl/gGGtT9 • ➜ LinkedIn: https://goo.gl/kSmbht • ➜ Google+: https://goo.gl/5bRNwC

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