Expression Vectors RNA Design Applications and Examples In vitro Transcription











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References/Resources:   / the_crux   • All videos on Genetic Engineering:    • Genetic Engineering (Genes to Genomes)   • RNA Expression vectors (different from protein expression vectors) are used for the sole purpose of making RNA from a template DNA. In this video, we discuss some of the common applications of RNA expression vectors and their rules/principles of design. Finally, we look at two examples, including in vitro transcription to understand how design principles and applications change our design of RNA expression vectors. • Jump to your favorite section: • 00:00 RNA Expression Vector • 01:00 Application • 02:00 Considerations • 04:35 Promoters and Terminators • 06:33 Example 1 • 08:43 In vitro Transcription • 11:45 Example 2 • References/Resources:   / the_crux   • Suggested videos: • Restriction Enzyme Cloning:    • Cloning with Restriction Enzymes (5 L...   • Bacterial Transcription Termination:    • Prokaryotic Transcription TERMINATION...   • Operons (lac, ara, trp):    • Gene Expression Regulation   • • Must watch playlists • All videos on Genetic Engineering:    • Genetic Engineering (Genes to Genomes)   • All videos on DNA Replication:    • All about DNA Replication!   • All videos on Transcription:    • All about Transcription!   • All videos on Translation:    • All about Translation!   • Consider supporting the growth of this channel by becoming a Patron:   / the_crux   • I appreciate your support :)

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