Expression Vectors RNA Design Applications and Examples In vitro Transcription
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References/Resources: / the_crux • All videos on Genetic Engineering: • Genetic Engineering (Genes to Genomes) • RNA Expression vectors (different from protein expression vectors) are used for the sole purpose of making RNA from a template DNA. In this video, we discuss some of the common applications of RNA expression vectors and their rules/principles of design. Finally, we look at two examples, including in vitro transcription to understand how design principles and applications change our design of RNA expression vectors. • Jump to your favorite section: • 00:00 RNA Expression Vector • 01:00 Application • 02:00 Considerations • 04:35 Promoters and Terminators • 06:33 Example 1 • 08:43 In vitro Transcription • 11:45 Example 2 • References/Resources: / the_crux • Suggested videos: • Restriction Enzyme Cloning: • Cloning with Restriction Enzymes (5 L... • Bacterial Transcription Termination: • Prokaryotic Transcription TERMINATION... • Operons (lac, ara, trp): • Gene Expression Regulation • • Must watch playlists • All videos on Genetic Engineering: • Genetic Engineering (Genes to Genomes) • All videos on DNA Replication: • All about DNA Replication! • All videos on Transcription: • All about Transcription! • All videos on Translation: • All about Translation! • Consider supporting the growth of this channel by becoming a Patron: / the_crux • I appreciate your support :)
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