TIMELAPSE Diferenciación de los embriones somáticos

>> YOUR LINK HERE: ___ http://youtube.com/watch?v=53UoBvjgzi8

Time-lapse of the differentiation of somatic embryos from grapevine. Starting from a suspension culture, the somatic embryos were sowed on a semi-permeable acetate cellulose membrane placed on differentiation medium. In the course of the video you can see how the embryos cross the different stages of differentiation: heart, torpedo and cotyledonary, progressively. • For the realization of this time-lapse, photos have been taken every hour from the beginning of the culture during for 32 days. Because the framerate is 24 fps, 1 second of video equals approximately 2 days in real time. • ------------------------------------------------------------------------------------- • To go into more detail (culture media, times, results, etc.), the references of the articles on which the whole process is based: • Acanda Y, Prado MJ, González MV, Rey M (2013) Somatic embryogenesis from stamen filaments in grapevine (Vitis vinifera L . cv . Mencía): changes in ploidy level and nuclear DNA content. In Vitro Cell Dev Biol Plant 49: 276–284. • Prado MJ, Grueiro MP, González MV, Testillano PS, Domínguez C, López M, Rey M (2010) Efficient plant regeneration through somatic embryogenesis from anthers and ovaries of six autochthonous grapevine cultivars from Galicia (Spain). Sci Hortic Amsterdam 125: 342–352. • ------------------------------------------------------------------------------------- • Music used in this video: • Fall of the Solar King by Twin Musicom (twinmusicom.org) • ------------------------------------------------------------------------------------- • Researchgate: https://www.researchgate.net/profile/... • Website of the research group: • http://mrey.webs.uvigo.es/Home.html

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