How To Run the Bolt PreCast Protein Gel

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Learn more at https://products.invitrogen.com/ivgn/... • How to run the Bolt Bis-Tris Plus Pre-cast Protein gel on the Bolt Mini-Gel Tank. • Do you run pre-cast protein gels? Are you doing any Western blotting? If you do, you should check out the New Bolt Pre-Cast Protein Gels and Bolt Electrophoresis Tank. The Bolt system is designed to give you best-in-class Western blots every time. • The new Bolt BIS-TRIS-PLUS Gels come in different concentrations and gradients, and you should pick the one that is best suited relative to the molecular weight of the proteins that you are working with, the sample volume, and the number of samples. All Bolt gels have wedge wells that allow you to load 50% more sample to each well. • You'll want to first place the Bolt tank stand on a flat benchtop surface, then snap the electrophoresis tank into the stand. • Then, you can place the cassette clamps in the appropriate chambers of the side-by-side Bolt electrophoresis tank. The Anode connectors should be positioned in the middle of the tank away from the Cathode connectors • You'll next need to cut open the gel cassette pouch and remove the gel cassette from the pouch. • Gently, Remove the tape at the lower portion of the cassette. • You can gently remove the comb by sliding the comb up one side at a time. Wash the cassette wells with 1X running buffer. Invert the gel and shake removing buffer. • Now, you can place the gel cassettes into the cassette clamps with the wells facing forward towards you. Secure the gel by closing the clamp. The lever should come to a stop and holds the gel firmly in place. • Now, you can fill electrophoresis tank with 1xBolt SDS Running Buffer, so the level is above the electrode, but not over the top of cassette. Completely fill the wells. • • Now, you're ready to prepare your protein samples with appropriate sample buffer. • The forward-facing wedge wells make it easy to load sample, lower the pipette tip into the wedge well and pipette sample into the well. • Just place the lid on top of the tank and align the electrodes over the corresponding banana plugs. • Connect the electrode cords to the power supply. • Turn on the power. • The Bolt™ tank is fast and easy to set-up with perfect no-leak seal results and no mess. Gone are the days of blobby protein signals, now enjoy straight, sharp protein bands with even greater sensitivity and resolution on every Western blot you run. You'll want to switch NOW

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