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Restriction Enzyme Digestion and Ligation
>> YOUR LINK HERE: ___ http://youtube.com/watch?v=6zJhruWqSBw
Restriction Enzyme Digestion and Ligation • Biochem 122 Activity 6 • Presented by: So and Sy (da seesaw gang) • Err on [Activity 6, Part II] Activity of a Novel RE X: • The optimum time should be 120 minutes where the band around 2700 bp is not present. • ------------------------------------------------------------------------------------------------------- • Time code: • 0:00 - Introduction • PART I: RESTRICTION ENZYME DIGESTION • 2:50 - [Activity 6.1a] Reading a Plasmid Map • 5:18 - [Activity 6.1b] Digesting a Plasmid • 8:20 - [Activity 6, Part II] Activity of a Novel RE X • 11:42 - [Activity 6, Part I] Guide Questions (A-D) • PART II: LIGATION • 14:27 - [Activity 6.2] Blunt-end and Sticky-end Ligation • 23:12 - [Activity 6, Part III] RE Digestion as a Powerful Screening Tool • 25:53 - [Activity 6.2, Part IV] Sticky-end Ligation with 2 Restriction Enzymes • 27:49 - [Activity 6, Part I] Guide Questions (E-F) • 28:52 - Conclusion and Recommendations
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