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Simple Detection Primary Cilia By Immunofluorescence l Protocol Preview
>> YOUR LINK HERE: ___ http://youtube.com/watch?v=7RcSZ1734XY
Watch the Full Video at https://www.jove.com/v/61155/simple-d.... • Simple Detection of Primary Cilia by Immunofluorescence - a 2 minute Preview of the Experimental Protocol • Alzbeta Filipova, Daniel Diaz Garcia, Josef Dvorak, Stanislav Filip, Marcela Jelicova, Zuzana Sinkorova • University of Defence, Department of Radiobiology, Faculty of Military Health Sciences in Hradec Kralove; University Hospital, Department of Clinical Biochemistry and Diagnostics; Charles University, Department of Oncology, Thomayer Hospital; Charles University, Department of Oncology and Radiotherapy, Faculty of Medicine; • Primary cilia are extracellular structures associated with the centriole. Primary cilia detection by immunofluorescent staining is a relatively simple procedure that results in extremely high-quality images. In this protocol, fibroblasts expressing primary cilia were fixed, immunostained, and imaged in a fluorescent or confocal microscope. • Visit https://www.jove.com?utm_source=youtube utm_medium=social_global utm_campaign=reseach-videos-2022 to explore our entire library of 14,000+ videos of laboratory methods and science concepts. • JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. • Subscribe to our channel: / jovejournalofvisualizedexperiments
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