Apoenzyme Cofactors Coenzymes and Holoenzymes quotShort Note 2minsquot











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This video explains about Apoenzyme, Cofactors, Coenzymes and Holoenzymes with examples. • Enzymes are biological catalysts, they speed up the biochemical reaction by reducing the activation energy. • The catalytic activity of many enzymes depends on the presence of small molecules called cofactors. • An enzyme without its cofactor is referred to as an apoenzyme; the complete, catalytically active enzyme is called a holoenzyme. • • Cofactors can be subdivided into two groups: • 1. Metal Ions and • 2. Small organic molecules • The enzyme Taq DNA polymerase require Mg2+ for its activity, Lactate dehydrogenase requires small organic molecule Nicotinamide adenine dinucleotide. • • Related Videos: • --------------------------------------------------------------------------------------------------------------- • Plotting Bacterial Growth Curve:    • Plotting Bacterial Growth Curve in Excel   • Colony Forming Units:    • CFU: Colony Forming Unit   cfu/ml cal...   • Serial Dilution Methods:    • Serial Dilution Methods   Calaculations   • Copy Number Calculation for qPCR:    • Copy Number Calculation for qPCR   • How to analyze Primer Sequence or Oligonucleotide sequence designed for PCR / qPCR:    • How to analyze Primer Sequence or Oli...   • How to generate qPCR standard curve in excel and calculate PCR efficiency:    • How to generate qPCR standard curve i...   • Real Time qPCR optimization, Calculating PCR Efficiency:    • Real Time qPCR  optimization, Calcula...   • Taqman Assay Vs SYBR Green Assay:    • Real Time PCR Assays- Taqman Assay Vs...   • Resolving poor PCR efficiency:    • Resolving Poor PCR assay Efficiency i...   • Calculating molecular weight of Unknown protein    • How to calculate molecular weight of ...   • Designing qPCR Primers •    • Primer designing for real time PCR us...   • Oligonucleotide Preparation Purification    • Oligonucleotide Preparation and Purif...   • How to check Oligo Concentration •    • How to check the Oligo concentration ...  

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