Basic SAXS Data Analysis and Validation

>> YOUR LINK HERE: ___ http://youtube.com/watch?v=8pgdRiEDGBQ

One of a series of lectures at the BioCAT Everything BioSAXS 9 workshop in February 2023. This lecture focuses on how to carry out basic data analysis and validation for small angle x-ray scattering (SAXS). It covers: • What a scattering profile is; • How poor sample quality, radiation damage, concentration, and bad buffer subtraction affects your data and how to diagnose these problems • Guinier fits to get radius of gyration (Rg) and scattering intensity at zero angle (I(0)) • Calculating molecular weight from SAXS data • Porod and Kratky analysis • Indirect Fourier transforms (IFTs) to get the pair-distance-distribution function (P(r)) • Note that this video is an updated version of the previous basic SAXS analysis video. • BioCAT (Sector 18, Advanced Photon Source): • https://www.bio.aps.anl.gov/ • More from Everything BioSAXS: • • Everything BioSAXS • Lecturer: • Jesse Hopkins • Beamline Scientist, BioCAT • Research Assistant Professor • Department of Physics • Illinois Institute of Technology • Slides: • https://www.bio.aps.anl.gov/files/eb5... • Date: February 28, 2023

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