MICROFLUIDIC BASED DIELECTROPHORESIS CELL SORTER finalyearprojects 2020

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Cancer is known to be the second leading cause of human death. It is a deadly disease that affects people throughout the world of any gender and any age. Mortality due to cancer is mainly due to late diagnosis and detection. Currently, there are few methods to detect cancer such as blood tests and biopsy. These methods come with subtle drawbacks which share similar disadvantages like huge time consumption to give a result of detecting stages of cancer. Dielectrophoresis (DEP) is a label-free method caused due to the non-uniform electric field. DEP is based on the parameters of cell properties such as size, capacitance, conductivity, and permittivity. The circulating tumor cells (CTCs) and red blood cells (RBCs) are different in size. So, the effect of the DEP force on them is different. This causes them to be located at different heights and flow rates, causing them to travel to different outlets. The study was carried out on simulation platform COMSOL Multiphysics by which the study on characterization of the design in terms of electric potential, pressure, fluid flow of the particles was carried out. The results contain two proposed designs of a microfluidic chip with array electrodes, consisting of two inlets and two outlets connected directly to the main channel. With the aid of dielectrophoresis field-flow-fractionation (DEP-FFF) using a low voltage of 10 Volts, the device was successful to separate cancerous cells from a mixture of CTCs and RBCs with an efficiency of 87% in 108 seconds. • #fyp • #dielectrics • Contact supervisor: Assoc. Prof. Ir. Dr. Mohd Ridzuan Bin Ahmad • [email protected] • 0:00 Introduction • 0:18 Introduction Incidence • 0:35 Problem Statement • 0:58 Project Objectives • 1:41 DEP-Field Flow Fractionation • 2:34 Results - Design Specifications • 3:12 Results - Parameters involved • 3:28 Design Justification • 4:26 Sutainability Study - Electric Potential • 5:04 Sutainability Study - Pressure • 5:26 Sutainability Study - Fluid flow velocity • 5:50 Sutainability Study - Voltage Applied • 6:15 Particle separation • 7:16 Conclusion

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