Western Blotting WB Video Protocol Series











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For more information, please visit https://www.stjohnslabs.com • Antibody Validation Project (Free Trial Size Antibodies) https://www.stjohnslabs.com/antibody-... • Western Blotting (WB) - Video Protocol:    • Western Blotting (WB) - Video Protoco...   • ImmunoHistoChemistry (IHC) - Video Protocol:    • ImmunoHistoChemistry (IHC) - Video Pr...   • Immunocytochemistry (ICC) - Video Protocol:    • Immunocytochemistry (ICC) - Video Pro...   • The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies. (From Wikipedia, the free encyclopedia)

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