VDJSeq Sequencing Analysis of Rearranged IgH Gene in BCell Lymphoma Protocol Preview

>> YOUR LINK HERE: ___ http://youtube.com/watch?v=BC98PiwK10s

Watch the Full Video at https://www.jove.com/v/53215/vdj-seq-.... • VDJ-Seq: Deep Sequencing Analysis of Rearranged Immunoglobulin Heavy Chain Gene to Reveal Clonal Evolution Patterns of B Cell Lymphoma - a 2 minute Preview of the Experimental Protocol • Yanwen Jiang, Kui Nie, David Redmond, Ari M. Melnick, Wayne Tam, Olivier Elemento • Weill Cornell Medical College, Department of Medicine; Weill Cornell Medical College, Institute for Computational Biomedicine; Weill Cornell Medical College, Department of Pathology and Laboratory Medicine; • This protocol describes an approach to interrogate the recombined immunoglobulin heavy chain VDJ regions of lymphomas by deep-sequencing and retrieve VDJ rearrangement and somatic hypermutation status to delineate clonal architecture of individual tumor. Comparing clonal architecture between paired diagnosis and relapse samples reveals lymphoma relapse clonal evolution modes. • Visit https://www.jove.com?utm_source=youtube utm_medium=social_global utm_campaign=reseach-videos-2022 to explore our entire library of 14,000+ videos of laboratory methods and science concepts. • JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. • Subscribe to our channel: / jovejournalofvisualizedexperiments

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