Amino acid and polypeptide synthesis 4

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Organic chemistry: Amino acid synthesis--Gabriel synthesis; Strecker synthesis. Edman degradation. Polypeptide synthesis--Cbz (carbobenzoxy) and Boc (tert-butoxycarbonyl) amino-protecting groups; protection of the carboxy terminus via ester formation; DCC (dicyclohexylcarbodiimide) carboxy-activating reagent. An example of calculating pI and charge at a specific pH for a long polypeptide • This is a recording of a tutoring session, posted with the students' permission. • These videos are offered on a pay-what-you-like basis. You can pay for the use of the videos at my website: • http://www.freelance-teacher.com/vide... • For a printable document containing the amino acid table discussed in this video series, go to my website. • For a list of all the available video series, arranged in suggested viewing order, go to my website. • For a playlist containing all the videos in this series, click here: • • Amino acid and polypeptide synthesis (1) • (1) Determining charge of an amino acid at a particular pH. Acidic vs. basic side chains. Gabriel synthesis of amino acids • (2) Gabriel synthesis continued • (3) Continued • (4) Continued. Strecker synthesis of amino acids • (5) Continued • (6) Continued • (7) Edman degradation • (8) Continued • (9) Continued. Polypeptide synthesis; Cbz (carbobenzoxy) amino-protecting group • (10) Cbz continued; Boc (tert-butoxycarbonyl) amino-protecting group • (11) Polypeptide synthesis continued; protection of the carboxy terminus via ester formation; DCC (dicyclohexylcarbodiimide) carboxy-activating reagent • (12) DCC continued • (13) An example of calculating pI and charge at a specific pH for a long polypeptide • (14) A polypeptide synthesis problem, illustrating the use of amino- and carboxy-protecting groups • (15) Continued • • tags: education college student students university exam test educational study campus school class

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