92 Amino Acids Peptides amp Proteins Bonding Sequencing and Analysis

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The ability of amino acids to polymerize into peptides and proteins is introduced. The nature of the peptide bond, amide group, is discussed. Methods for determining peptide structure are presented. N-terminal residue analysis, the Edman Degradation, and C-terminal residue analysis using carboxypeptidase are presented. Enzymatic cleavage of peptides by the enzymes chymotrypsin, pepsin, trypsin, and thermolysin as well as BrCN and BrCNanh is also discussed. • 00:00 Amino Acids • 00:12 Peptide Bond • 04:20 Peptide Nomenclature • 05:52 Implications of Peptide Bonding • 09:25 Oligopeptides • 10:49 Polypeptides Proteins • 12:24 Determination of Peptide Structure • 15:38 The Edman Degradation • 24:18 C-Terminus Analysis: Carboxypeptidase • 28:15 Analysis via Partial Hydrolysis • 32:20 Analysis via Enzymatic Cleavage • 33:16 Chymotrypsin • 37:19 Pepsin • 38:16 Trypsin • 38:57 BrCN (Aqueous Anhydrous) • 40:15 Thermolysin • 41:24 Sample Problem • 43:58 Cysteine Disulfide Bridges • 46:03 Bovine Oxytocin • 47:48 Bovine Insulin

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