Alignment of the laser position and adjustment of the photodetector position











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Inserted images on the right bottom is the 4-digit indicators for the total intensity of laser irradiated onto the four segments of the quadrant PIN photodiode (left) and difference between the laser intensities irradiated on the top two segments and the bottom two segments (right). Full protocol described in: • Takayuki Uchihashi, Noriyuki Kodera Toshio Ando. Guide to video recording of structure dynamics and dynamic processes of proteins by high-speed atomic force microscopy. Nature Protocols 7, 1193-1206 (2012) doi:10.1038/nprot.2012.047 • http://dx.doi.org/10.1038/nprot.2012.047

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