Youtor
In Situ Hybridization for BEGINNERS
>> YOUR LINK HERE: ___ http://youtube.com/watch?v=Bt0LiADEGHw
In situ hybridization is a technique used by scientists to label DNA and RNA, but why do we need to label DNA/RNA? • Here I explain the purpose of In Situ Hybridizations, provide a brief description of the two major types (chromogen and fluorescence in situ hybridization (FISH)), give several examples of their use, and explain some drawbacks. • This is a part of my Slice of Science series where I briefly describe some interesting scientific techniques. • Items used in the video: • Music: • Child of Time (1 HOUR) LOFI HIPHOP | ... • Articles used as examples: • Cancer Cell DNA Translocation Example • Louzada S, Adega F, Chaves R. Defining the sister rat mammary tumor cell lines HH-16 cl.2/1 and HH-16.cl.4 as an in vitro cell model for Erbb2. PLoS One. 2012;7(1):e29923. doi: 10.1371/journal.pone.0029923. Epub 2012 Jan 10. PMID: 22253826; PMCID: PMC3254647. • Figure 2A • Dentate Gyrus/Low Level Chromogen RNA In situ • Caramello A, Galichet C, Rizzoti K, Lovell-Badge R. Dentate gyrus development requires a cortical hem-derived astrocytic scaffold. Elife. 2021 Jan 4;10:e63904. doi: 10.7554/eLife.63904. PMID: 33393905; PMCID: PMC7806271. • Figure supplement 5A • Enthesis Example/Double RNA FISH • Kult S, Olender T, Osterwalder M, Markman S, Leshkowitz D, Krief S, Blecher-Gonen R, Ben-Moshe S, Farack L, Keren-Shaul H, Salame TM, Capellini TD, Itzkovitz S, Amit I, Visel A, Zelzer E. Bi-fated tendon-to-bone attachment cells are regulated by shared enhancers and KLF transcription factors. Elife. 2021 Jan 15;10:e55361. doi: 10.7554/eLife.55361. PMID: 33448926; PMCID: PMC7810463. • Figure 1A, Figure 2C • These articles are distributed under the terms of the Creative Commons Attribution License (CC-BY-4.0), which permits unrestricted use and redistribution provided that the original author and source are credited. • https://creativecommons.org/licenses/... • https://creativecommons.org/licenses/...
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