Youtor
How to Design primers using Primer3
>> YOUR LINK HERE: ___ http://youtube.com/watch?v=CgEr4Hg8Px4
how to make primers using Primer3 • 1.Go to https://genome.ucsc.edu/ • 2.Click on Genome browser • 3.write your gene name e.g BRSK1 • 4.click on Submit • 5.click on Ensembl • 6.In Ensembl click on sequence (left corner) • 7.you can see sequence on your screen • 8. choose 22 basepair (interon) before and after exons region • (brown highlighted region is exon) • 9.copy and go to http://bioinfo.ut.ee/primer3-0.4.0/ • 10.paste that sequence in bar • 11.use { in start and } in end • 12.click on pick primers..... done!
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