Precipitation Reactions of Proteins Biochemistry

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This video features Precipitation reactions of proteins • Proteins are large molecules with variable sizes, shapes and charges. They can be classified as simple, conjugated and derived proteins. Most simple proteins, especially globular proteins, when dissolved in water, form colloidal solution. A colloid is a system in which the particles have diameters in the range of 1 mm to about 200 mm. The stability of a solution of a lyophobic colloid depends on the electrical charges on the surface of particles, which prevent their coagulation and precipitation. In case of lyophilic colloids, over and above the surface charges, the degree of hydration (shell of water molecules around the particles) also contributes to the stability. Polar groups of the proteins (-NH2, COO–, OH– groups) tend to attract water molecules towards them to produce a shell of hydration. Albumin has a greater degree of hydration than globulins. Purification of enzymes and other proteins usually start with precipitating them from solution. Any factor, which neutralises the charge or removes water of hydration will therefore cause precipitation of proteins. These characteristics of protein molecules depend upon their molecular weights, three dimensional structures and properties of the constituent amino acids. • Different methods of precipitation • -Saturation test • -Isoelectric ph • -organic solvent • -mineral acids • -Heavy metal cations • -esbachs reagent • be safe in home during this pandemic season and try to watch all my videos. • This video has been recorded and edited with much effort and difficulty. Lack of proper equipment and chemicals can make video look unfinished. I request your love and support in donations also. • Links below 👇 • Support me on patreon • / vibzzlab • Or • Support me via paypal • https://www.paypal.me/vibzzlab • connect with me on social media • / vibzz_lab • • https://twitter.com/vibzzlab?s=09 • • / about • If you have any suggestions on new videos , modification to be done or queries regarding any content or anything you don't understand or complaints regarding any of the contents, • You can email me • Email id : [email protected] • Intro music courtesy. • Lights by Sappheiros / sappheirosmusic • Creative Commons — Attribution 3.0 Unported — CC BY 3.0 • Free Download / Stream: https://bit.ly/LightsSappheiros • Music promoted by Audio Library • Lights – Sappheiros (No Copyright Music) • rest of the video • youtube free audio library

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