BH3 Profiling A Flow Cytometry Protocol to Determine Apoptotic Competency

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Note: This seminar series has permanently moved to the EMGSS YouTube channel. For more videos in this series, visit: / @emgssmcgilluniversity9527 • This technical seminar was presented by Ryan Rys, a PhD candidate in the Johnson lab at the Lady Davis Institute on December 16th, 2020. This seminar is part of a biomedical research technical seminar series hosted by McGill University's Experimental Medicine Graduate Students' Society (EMGSS), Graduate Association of Physiology (GAP) and Graduate Association of Pharmacology and Therapeutics Students (GAPTS). See emgss.org/tss for more. • A hallmark of cancer growth and progression is the evasion of apoptosis and resistance to treatment in malignant cells. Recent focuses of cancer research have included the development of targeted therapies aimed at increasing a cell’s response to apoptotic stimuli. This seminar highlighted a technique for analyzing apoptosis in cancer cells called BH3 profiling. This flow cytometry assay helps to determine whether a cell can undergo apoptosis by assessing the balance between pro- and anti-apoptotic proteins. A fast and efficient method, Ryan discussed the protocol and provided examples of how BH3 profiling can be used to classify levels apoptotic dysfunction, screen for drugs that are efficient in inducing cell death and identify potential therapeutic targets.

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