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Northern blotting technique
>> YOUR LINK HERE: ___ http://youtube.com/watch?v=GBmFaf2krYY
Northern blotting technique step by step - This lecture explains about the northern blotting procedure and technique to identify specific RNA fragment from a mixture of cellular RNA molecules separated by the formaldehyde treated Agarose gel electrophoresis. Northern blotting step by step explanation is provided to understand the procedure of the RNA separation and identification. The difference between Southern blotting and northern blotting is also explained in this video. • Northern blotting consists of several different stages - • 1. First is the isolation of mRNA from the target cell. • 3. Then the separation of the native RNA molecules using formaldehyde treated Agarose gel electrophoresis so that all the RNA gets linearity. • 4. Transfer of the Agarose gel RNA bands to a nitrocellulose or nylon Membrane filter. This filter paper after the RNA transfer is known as northern blot. • 5. Then the use of complementary DNA or cDNA probe to bind with the target RNA that will indicate the presence of target sequence by radioactivity or by colorimetric analysis. Northern blotting was invented in 1979 at it was named after the southern blotting technology to identify RNA using blotting technique. • For more information, log on to- • http://www.shomusbiology.com/ • Get Shomu's Biology DVD set here- • http://www.shomusbiology.com/dvd-store/ • Download the study materials here- • http://shomusbiology.com/bio-material... • Remember Shomu’s Biology is created to spread the knowledge of life science and biology by sharing all this free biology lectures video and animation presented by Suman Bhattacharjee in YouTube. All these tutorials are brought to you for free. Please subscribe to our channel so that we can grow together. You can check for any of the following services from Shomu’s Biology- • Buy Shomu’s Biology lecture DVD set- www.shomusbiology.com/dvd-store • Shomu’s Biology assignment services – www.shomusbiology.com/assignment -help • Join Online coaching for CSIR NET exam – www.shomusbiology.com/net-coaching • We are social. Find us on different sites here- • Our Website – www.shomusbiology.com • Facebook page- / shomusbiology • Twitter - / shomusbiology • SlideShare- www.slideshare.net/shomusbiology • Google plus- https://plus.google.com/1136485849827... • LinkedIn - / suman-bhattacharjee-2a051661 • Youtube- / thefunsuman • Thank you for watching Northern blotting lecture.
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