H amp E Staining Hematoxylin and Eosin stain Histopathology Hindi

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In This video, I have explained about Hematoxylin Eosin stain of the tissue section. about basics of H E stain and science behind it. • H E stain is most common and popular stain in histology. it is done on tissue section obtained on slide from processing of a biopsy sample. • H and E staining Procedure steps- • it includes - 1.dewaxing or deparaffinization • 2.hydration • 3. nuclear stain by hematoxylin (Harris's ) • 4. differentiation by acid alcohol • 5. Bluing • -6. counter stain by eosin • 7. dehydration • 8. clearing and mounting • 9.Result Interpretation • Summary - • 1.Hematoxylin Staining: Hematein/aluminum binds to negatively charged molecules (nucleic acids) in cell and tissues • 2. Differentiation Step: Uses acidic solution to remove excess of hematoxylin or/and background staining • 3. Bluing Step: Necessary to convert nuclear coloration from reddish purple to a crisp blue/purple • 4 Eosin Staining: Eosin binds to positively charged proteins in • the cytoplasm, connective tissue, muscle and red blood cells staining them different shades of red or pink. • For Regular Update join us at Instagram page - / paramedical_nursing_academiayt • Disclaimer- If Some contents are used for an educational purpose under fair use. Copyright Disclaimer Under Section 107 of the Copyright Act 1976, allowance is made for fair use for purposes such as criticism, comment, news reporting, teaching, scholarship, and research. All credit for copyright material used in the video goes to the respected owner.

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