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Primerdesign qPCR Tips Technical Replicates
>> YOUR LINK HERE: ___ http://youtube.com/watch?v=HvQMH9I3Avg
Welcome to Primerdesign qPCR Tips: • Technical replicates are an essential requirement; that means running two or more identical wells for each data point. • For most routine work, running duplicate wells is perfectly adequate. • However if you are working on very low copy targets then three might be necessary to give added confidence to your measurements. • This means if one reaction fails the outlier can be removed and there are still 2 data points to take an average of. • You are looking for your technical replicates to come within half a Cq value of each other. • Visit our Website http://www.primerdesign.co.uk/home
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