Drosophila oogenesis Intermediate Guide 23











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00:16 Beginning • 00:39 Germline stem cells (GSC), stem cell niche, cap cells, decapentaplegic, escort cells • 03:04 Cystoblast generates germline cyst, incomplete divisions, ring canals, cytoplasmic bridge • 04:06 Why number of ring canals per cell (cystocyte) is important, invariant pattern • 06:36 Pro-oocytes, oocyte • 07:38 (Pro-) Nurse cells in more detail • 09:21 Pro-oocytes and MTOC/Centrosome • 10:52 Oocyte and MTOC/Centrosome • 12:36 Fusome, Spectrosome: How pro-oocytes are selected. Directing cell-division plane. Ring canals. Oocyte Selection. • 21:38 Oocyte Selection - Cell Competition Model, Early Asymmetry Model • 27:05 Balbiani body • 28:28 Quick summary of things we just discussed, outlook for part 3 • 29:32 Three important pathways for germline-follicle cell interaction: (1) Delta-Notch, (2) JAK/STAT, (3) Gurken-Torpedo (TGF-alpha, EGFR) • • Sources: • (Refers to all parts of this series) • (1): Symmetry Breaking During Drosophila Oogenesis: Siegfried Roth, Jeremy A. Lynch, 2009 • (2): The Centrosome in Drosophila Oocyte Development: Timothy L. Megrow, Thomas C. Kaufman, 2000 • (3): Modulation of Cell-Cell Interactions in Drosophila Oocyte Development: Antel, Inaba, 2020 • (4): Coordinating Proliferation, Polarity, and Cell Fate in Drosophila Female Germline: Hinnant, Merkle, Ables, 2020 • (5): Drosophila oogenesis: R. Bastock, D. St. Johnston, 2008 • (6): Drosophila oocyte localization is mediated by differential cadherin-based adhesion: Godt, Tepass, 1998 • (7): The Origin of Asymmetry: Early Polarisation of the Drosophila Germline Cyst and Oocyte: Huynh, Johnston, 2004 • (8): Drosophila follicle cells are patterned by multiple levels of Notch signalling and antagonism between the Notch and JAK/STAT pathways: Kunik, Torres, 2007 • (9): Drosophila follicle cells: morphogenesis in an eggshell: Wu, Tanwar, 2008 • (10): Organizer activity of the polar cells during Drosophila oogenesis: Grammont, Irvine, 2002 • (11): Drosophila oogenesis: Coordinating germ line and soma: Siegfried Roth, 2001 • (12): Notch Signaling during Oogenesis in Drosophila melanogaster: Xu, Gridley, 2012 • (13): Notch-Delta signaling induces a transition from mitotic cell cycle to endocycle in Drosophila follicle cells – Deng, Althauser, 2001 • (14): Delta signaling from the germ line controls the proliferation and differentiation of the somatic follicle cells during Drosophila oogenesis: Lopez-Schier, Johnston, 2001 • (15): Pattern Formation: Gurken meets torpedo for the first time: Elizabeth R. Gavis, 1995 • (16): JAK/STAT Pathway Signalling in Drosophila Melanogaster: S. Bina, M. Zeidler, 2009 • (17): At the crossroads of differentiation and proliferation: Precise control of cell-cycle changes by multiple signaling pathways in Drosophila follicle cells: S. Klusza, W. Deng, 2011 • (18): A Gene Expression Screen in Drosophila melanogaster Identifies Novel JAK/STAT and EGFR Targets During Oogenesis: J. Wittes, T. Schüpbach, 2019 • (19): The Centrosome in Drosophila Oocyte Development: T. L. Megraw, T. C. Kaufman, 2000 • (20): Eggs to die for: cell death during Drosophila oogenesis: M. Buszczak, L. Cooley, 2000 • (21): Developmental Biology: Gilbert, Barresi, 2016 • (22): Principles of Development: L. Wolpert, 2007

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