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Recombinant DNA technology NMDCAT 2021
>> YOUR LINK HERE: ___ http://youtube.com/watch?v=Kh9Z-nj6qYo
Hi Everyone! Welcome To My Channel Ali Academy Biology Lectures • About This Video ........................ • In This Video Lecture You Will Learn Basic Concept Of Recombinant DNA Technology • Recombinant DNA technology popularly known as genetic engineering aims at • 1. Gene of interest, which is to be cloned. • 3. Molecular carrier or vector, on which gene of interest could be placed. • 4. The gene of interest along with the vector is then introduced into an expression • order to produce recombinant DNA, the following are required: • synthesizing recombinant DNA which contains DNA from two different sources. In • 2. Molecular scissors to cut out the gene of interest. • system, as a result of which a specific product is made. • How to get a gene? • cut the DNA at very speciic sites characterized by speciic sequence of four or six • nucleotides arranged symmetrically in the reverse order. Such sequences are known • • which a piece of foreign DNA can be placed, if it ends in bases complementary to those • Another very common method of getting the gene is to synthesize it in the laboratory • growth of viruses. In 1970, Hamilton O. Smith, at Johns Hopkins University, isolated the • as palindromic sequences. So far more than 400 such enzymes have been isolated out • If, however, the genes are small, they can also be synthesized in the laboratory. • Genes can be isolated from the chromosomes by cutting the chromosomes on the • EcoRl, a commonly used restriction enzyme, cuts double-stranded DNA when it has • exposed by the restriction enzyme. The single stranded but complementary ends of the • complementary DNA (cDNA). • lanking sites of the gene using special enzymes known as restriction endonucleases. • (b) to synthesize it chemically, and • These are natural enzymes of bacteria, which they use for their own protection against • (c) to make it from mRNA • viruses. The restriction enzyme cuts down the viral DNA, but does no harm to the • of which about 20 are frequently used in recombinant DNA technology. • this sequence of bases at the cleavage site . Notice there is now a gap into • bacterial chromosome. They are called restriction enzymes because they restrict the • (a) to isolate it from the chromosome • Molecular Scissors: Restriction Endonucleases • from messenger RNA, using reverse transcriptase. This DNA molecule is called • There are three possible ways to get the gene of interest. • two DNA molecules are called “sticky ends” because they can bind by complementary • irst restriction enzyme. Bacteria produce a variety of such restriction enzymes, which • base pairing. They, therefore, facilitate the insertion of foreign DNA into vector DNA. • Molecular Carrier: Vector • antibiotic resistance gene for tetracycline, whereas pBR 322 has antibiotic resistance • To make recombinant DNA, one often begins by selecting a vector, the means by • antibiotic resistance and fertility etc. One of the plasmids discovered earlier pSC 101 has • which recombinant DNA is introduced into a host cell. One common type of vector • is a plasmid. Plasmids were discovered by investigators studying the sex life of the • Plasmids are natural extra-chromosomal circular DNA molecules which carry genes for • intestinal bacterium Escherichia coli. • • genes for tetracycline as well as ampicillin. Inserting gene of interest in tetracycline • resistant gene of plasmid pBR 322 would enable separating out colonies of bacteria in • a medium containing ampicillin and vice versa. • About This Channel..................... • I make these videos cause I love to draw and connect the complexity of science and medicine into art. I'm not saying. I'm 100% correct in all my videos, but I do try to obtain the information from credible sources. • One-stop destination for all biology lectures. • _ students in class 11, 12 or appearing for competitive medical exams ( NMDCAT ) will find it very beneficial • _ The videos are categories under various heading to make the students life simplified • Visit My Other Channel Playlist ..................... • NMDCAT PART 1: • New 1st Year Biology • NMDCAT PART 2: • New 2nd Year Biology • Class 11th Lectures: • New 1st Year Biology • Class 12th Lectures: • New 2nd Year Biology • Class 10th Lectures: • • Matric Biology • Subscribe My Channel: • / aliacademya7r • Social Link........................ • Instagram: • https://instagram.com/ranaalibiologis... TWINS
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