The Basics of Lentivirus ProductionPackaging Protocol Tips and more

>> YOUR LINK HERE: ___ http://youtube.com/watch?v=LzgsgVO5WYI

Want to package recombinant lentiviruses? What packaging cell line should you use? How do you achieve higher titers? How do you harvest your lentiviruses after they’ve been packaged? • Lentiviruses are a key tool in today’s field of biology as they provide a reliable way to achieve stable over-expression of a gene in your cells of interest. However, many scientists are confused about how to produce these genetic engineering marvels in their own labs. • In this video, we'll outline the steps/protocol for packaging lentiviruses, including: • • Considerations before you start [1:02] • • Basic packaging protocol [01:33] • • Storing your lentiviruses [4:19] • • Infection test experiment [4:56] • • Additional tips and tricks for lentivirus production • After watching this video, you’ll be well on your way to becoming a lentivirus production champion! • [4:05] Note: An error in pronunciation. It is a 0.45 µm (micron) filter (not micromolar) that is recommended for use. • You can read more about how to package lentiviruses and get a hard copy of the protocol here: • ➜ https://info.abmgood.com/lentivirus-s... • Connect with us on our social media pages to stay up to date with the latest scientific discoveries: • ➜ Facebook: / appliedbiological.materials • ➜ Twitter: / abm_good • ➜ LinkedIn: / applied-biological-material • ➜ Instagram: / abm_good • Background music credit: Back To Portland by TrackTribe

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