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SDSPolyacrylamide Gel Electrophoresis Part II
>> YOUR LINK HERE: ___ http://youtube.com/watch?v=MILiO1XnuqQ
Hey Friends, • SDS-PAGE (Sodium Dodecyl Sulfate PolyAcrylamide Gel Electrophoresis) is used to separate proteins in a sample according to their molecular weight. • SDS binds to linearised proteins and masks the native charge. 1.4 g SDS bind per 1g protein, so that all proteins have a nearly similar mass-to-charge ratio. • After an SDS-PAGE, the gel is frequently used for Western Blot analysis to confirm the presence of a specific protein of interest. • Western Blot: • • Western Blot / Protein Immunoblot exp... • Here is a helpful explanation on SDS-PAGE: • https://ruo.mbl.co.jp/bio/e/support/m... • Thanks Bye • Henrik
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