Youtor
Acrolein test
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PURPOSE OF TEST: • This test is used to identify pure glycerol. Glycerol is a trihydric alcoholic lipid. It occurs in state in combine state as glycerol esters. Glycerol in lipids makes esters with fatty acids. • • Acrolein test • PURPOSE OF TEST: • This test is used to identify pure glycerol. Glycerol is a trihydric alcoholic lipid. It occurs in state in combine state as glycerol esters. Glycerol in lipids makes esters with fatty acids. • REAGENTS GLASSWARES: • 1. Solid potassium hydrogen sulphate • 2. Pure glycerol • 3. Test tubes, test tube stands, test tube holder, distilled water, pipette with sucker, beakers and graduated cylinder. • PROCEDURE: • 1. Take two neat test tubes and label them T (test sample) and C (control). • 2. Add 3 drops of distilled water in test tube C and 3 drops of glycerol in test tube T with the help of pipette. • 3. Add pinch of solid potassium hydrogen sulphate in both test tubes and mix well and heat strongly over small flame. • INTERPRETATION: • • A pungent smell in test tube T indicates the presence of glycerol in test tube T. • • No pungent smell is formed in the test tube C containing water shows that results are negative. • PRINCIPLE: • On heating with potassium hydrogen sulphate the glycerol become dehydrated to produce dehydrated aldehyde which is called as acrolein with pungent smell. This is because KHSO4 is strong dehydrating agent.
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