Immunoprecipitation and ABE Protein Palmitoylation Protocol Preview

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Watch the Full Video at https://www.jove.com/v/50031/detectio.... • Detection of Protein Palmitoylation in Cultured Hippocampal Neurons by Immunoprecipitation and Acyl-Biotin Exchange (ABE) - a 2 minute Preview of the Experimental Protocol • G. Stefano Brigidi, Shernaz X Bamji • University of British Columbia , Department of Cellular and Physiological Sciences, Brain Research Centre; • The reversible addition of palmitate to proteins is an important regulator of intracellular protein trafficking. This is of particular interest in neurons where many synaptic proteins are palmitoylated. We utilize a simple biochemical method to detect palmitoylated proteins in cultured neurons, which can be adapted for multiple cell types and tissues. • Visit https://www.jove.com?utm_source=youtube utm_medium=social_global utm_campaign=reseach-videos-2022 to explore our entire library of 14,000+ videos of laboratory methods and science concepts. • JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. • Subscribe to our channel: / jovejournalofvisualizedexperiments

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