Ribonuclease RNAse Properties of RNases RNAse Contamination and Detecting RNase Contamination

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This video explains about Ribonuclease [RNAse] : Properties of RNases, RNAse Contamination and Detecting RNase Contamination. • RNase, Ribonuclease is a type of nuclease that catalyzes the degradation of RNA into smaller components. • RNAse A (13,686 Da) is an endonuclease that cleaves single-stranded RNA at cytosine (C) or uracil (U) residues, producing 3'-phosphate termini. • Rnases are found in all cell types and organisms from prokaryotes to eukaryotes. • They are found in air and surface of the body and can contaminate through touching the surfaces. • Rapid RNase detection is achieved using a cleavable fluorescent-labeled RNase substrate. • RNAse Alert: • https://www.idtdna.com/pages/products... • Related Videos: • --------------------------------------------------------------------------------------------------------------- • Plotting Bacterial Growth Curve: • Plotting Bacterial Growth Curve in Excel • Colony Forming Units: • CFU: Colony Forming Unit cfu/ml cal... • Serial Dilution Methods: • Serial Dilution Methods Calaculations • Copy Number Calculation for qPCR: • Copy Number Calculation for qPCR • How to analyze Primer Sequence or Oligonucleotide sequence designed for PCR / qPCR: • How to analyze Primer Sequence or Oli... • How to generate qPCR standard curve in excel and calculate PCR efficiency: • How to generate qPCR standard curve i... • Real Time qPCR optimization, Calculating PCR Efficiency: • Real Time qPCR optimization, Calcula... • Taqman Assay Vs SYBR Green Assay: • Real Time PCR Assays- Taqman Assay Vs... • Resolving poor PCR efficiency: • Resolving Poor PCR assay Efficiency i... • Calculating molecular weight of Unknown protein • How to calculate molecular weight of ... • Designing qPCR Primers • • Primer designing for real time PCR us... • Oligonucleotide Preparation Purification • Oligonucleotide Preparation and Purif... • How to check Oligo Concentration • • How to check the Oligo concentration ...

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