Subculture of a Cell Line











>> YOUR LINK HERE: ___ http://youtube.com/watch?v=PC51Z5FKZXQ

As cultured cells grow and divide, they use up nutrients and eventually, cells will multiply to a point that they run out of space in the culture vessel. • Subculturing is the process of diluting and transferring the cells into more vessels with fresh culture medium so we can produce a new culture and the cells can continue growing. • In this video, we demonstrate the steps for propagating a culture by transferring NIH3T3 cells from a previous culture to a new culture vessel. • --------- • • Explore our free interactive eLearning tools at http://ncbionetwork.org/iet.

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