Assess qPCR Efficiency Using a 10Fold Serial Dilution

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To optimize your qPCR conditions, try varying conditions such as annealing temperature or primer concentration Then use a 10-fold serial dilution to check the efficiency of your qPCR. An example of this can be found in our AAV Titration by qPCR protocol: • https://www.addgene.org/protocols/aav... •    • AAV Titration by qPCR  

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