Measuring Proteins ELISA IQELISA SIMOA

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White Paper: https://www.raybiotech.com/elisa-immu... • Technology Platforms: • ELISA: https://www.raybiotech.com/elisa-kits/ • Immuno-PCR: https://www.raybiotech.com/elisa-kits... • SIMOA: https://www.raybiotech.com/cro-servic... • Speaker: Brittany: / bmpope • The basic design principle of ELISA depends on the stability and specificity of antibody-antigen interactions. Briefly, a capture antibody that is immobilized to a solid substrate, such as a bead or microplate, binds to a protein-of-interest in a variety of sample types. Such samples may include plasma, serum, cell and tissue lysates, conditioned media, cerebrospinal fluid, or urine. When ELISA was first introduced in 1971, an enzyme-labeled antigen was captured by an antibody on cellulose particles. • The antibody-antigen conjugate was then isolated through repeated centrifugation and washing. Since then, different ELISA formats have been developed, including the sandwich ELISA where a target protein is “sandwiched” between a capture antibody and a labeled detection antibody. This ELISA format is highly specific since two antibodies are required to bind to a protein for detection. Moreover, protein concentrations (i.e., quantitative data) can be determined with a standard curve. The ELISA platforms discussed here – standard ELISA, immuno-PCR, and single molecule array (SIMOA) – are sandwich ELISAs that generate quantitative data. However, they differ in terms of their substrates, detection methods, instruments, sample volumes, and sensitivities. • 0:00 Sandwich ELISA development • 1:05 Standard ELISA • 4:06 IQELISA • 6:35 SIMOA • 9:00 Multiplexing

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