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LPyrrolidonyl Arylamidase PYR Test PYR Test PYR Test In Microbiology
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The optochin susceptibility test is a diagnostic tool used to identify Streptococcus pneumoniae (pneumococcus) and distinguish it from other alpha-hemolytic streptococci. • Here's how the test works: • 1. A small amount of optochin (ethylhydrocupreine hydrochloride) is placed on an agar plate. • 2. A sample of the bacterial culture is streaked onto the plate. • 3. The plate is incubated at 37°C (98.6°F) for 24-48 hours. • 4. If the bacteria are susceptible to optochin, they will be inhibited or killed, resulting in a zone of inhibition around the optochin disc. • 5. The diameter of the inhibition zone is measured to determine the degree of susceptibility. • Interpretation: • Zone diameter ≥ 14 mm: Susceptible • Zone diameter 11-13 mm: Intermediate • Zone diameter ≤ 10 mm: Resistant • The optochin susceptibility test is a valuable tool for: • Identifying S. pneumoniae • Guiding antibiotic therapy • Monitoring antibiotic resistance • Keep in mind that this test is just one part of a comprehensive diagnostic approach, and results should be interpreted in conjunction with other laboratory findings and clinical information. • #optochin
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