Youtor
Ready Set Replicate
>> YOUR LINK HERE: ___ http://youtube.com/watch?v=U3InbXpv86o
Although biologists have been watching cells divide under the microscope for ~150 years, visualizing the steps of interphase in live cells has been difficult. In 2008 Sakaue-Sawano et al. developed a clever method, called FUCCI imaging, to watch a critical decision during interphase - the choice to start replicating DNA. • In FUCCI imaging, red and green fluorescent proteins are fused to two interphase regulators, Cdt1 and Geminin, respectively. Cdt1 exists only during G1 and early S phase, whereas Geminin exists during S/G2. Thus, cells appear red in G1, yellow in early S, and green in late S and G2 phases. Here human fibroblasts are visualized by time-lapse live-cell imaging (phase-contrast and fluorescent images acquired every 15 min with 10x objective). • Video courtesy of Teresa Davoli, Eros Lazzerini Denchi, and Titia de Lange, The Rockefeller University. • See more cell cycle images in the Cell Picture Show: https://www.cell.com/pictureshow/cell...
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