How to Do a Gram Stain Biology Lab Techniques

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Gram staining is a method of staining used to distinguish and classify bacterial species into two large groups (gram-positive and gram-negative). The name comes from the Danish bacteriologist Hans Christian Gram, who developed the technique. • 00:00 - Gram Stain description • 00:37 - Heat-fixation • 00:53 - Reagents description • 01:05 - Crystal violet application • 01:30 - Lugol's reagent application • 01:52 - Ethanol/Acetone wash • 02:19 - Safranin application • 02:41 - Drying • 02:44 - Microscopic observation • Video produced by Dr Guillaume Erard ‪@guillaumeerard‬ • Techniques demonstrated by Ms Ayumu Nishimura • Learn more on http://www.stmaur.ac.jp/ • #microbiology #gramstain #ibbiology

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