IsoView Wholebrain functional imaging in larval zebrafish

>> YOUR LINK HERE: ___ http://youtube.com/watch?v=U_NvQ2g6dGc

IsoView whole-brain functional imaging in larval zebrafish. • Dorsoventral (left) and rotating (right) maximum-intensity projections of a multi-view deconvolved IsoView time-lapse recording of the brain of a 3-day old larval zebrafish expressing the calcium indicator GCaMP6s throughout its nervous system. Functional imaging was performed at 1 Hz. The video captures several instances of motor-related, large-scale brain activity in the awake animal. The color code shows low and high fluorescence intensity levels in blue and green, respectively. (Video 5). • Within less than a second, the new IsoView microscope produces images of entire organisms, such as a zebrafish or fruit fly embryo, with enough resolution in all three dimensions that each cell appears as a distinct structure. • Video: Keller Lab, HHMI/Janelia Research Campus • News story: https://www.hhmi.org/news/seeing-big-... • Visit the Keller Lab at Janelia: https://www.janelia.org/lab/keller-lab

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