Detection Nitric Oxide amp Superoxide Radical Anion from Cells l Protocol Preview
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Watch the Full Video at https://www.jove.com/v/2810/detection.... • Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps - a 2 minute Preview of the Experimental Protocol • Bhavani Gopalakrishnan, Kevin M. Nash, Murugesan Velayutham, Frederick A. Villamena • The Ohio State University, The Davis Heart and Lung Research Institute; College of Medicine, The Ohio State University, Department of Pharmacology; • Electron paramagnetic resonance (EPR) spectroscopy was employed to detect nitric oxide from bovine aortic endothelial cells and superoxide radical anion from human neutrophils using iron (II)-N-methyl-D-glucamine dithiocarbamate, Fe(MGD)2 and 5,5-dimethyl-1-pyroroline-N-oxide, DMPO, respectively. • Visit https://www.jove.com?utm_source=youtube utm_medium=social_global utm_campaign=reseach-videos-2022 to explore our entire library of 14,000+ videos of laboratory methods and science concepts. • JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. • Subscribe to our channel: / jovejournalofvisualizedexperiments
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