Advanced Unmixing in SpectroFlo Cytek Aurora

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How to decide between cells controls and beads controls. • Video Contents: • 0:00 Intro • 1:18 Unmix with all beads • 2:09 Setting gates on beads in the unmixing wizard • 5:56 Setting up worksheets to check unmixing • 12:38 Determine which beads controls caused unmixing errors • 23:39 Unmixing a second time with a combination of beads and cells • 25:37 Setting gates on cells in the unmixing wizard • 31:04 Assessing unmixing quality after using beads and cells • 31:38 Troubleshooting unmixing errors • 35:02 Final checks if there are no unmixing errors • 36:00 Applying compensation to make final minor adjustments • 39:33 Outro

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