GRAM POSITIVE VS GRAM NEGATIVE BACTERIA bacteria biologyclass12 peptidoglycan











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GRAM POSITIVE VS GRAM NEGATIVE BACTERIA • Bacteria have cell walls made up of polysaccharides that give them strength and rigidity. Nearly all bacterial cell walls have a peptide-polysaccharide layer called peptidoglycan, or sometimes murein. • Peptidoglycan is a polymer made up of sugars and amino acids which forms a kind of mesh. Bacteria can be classified based on their reaction to the Gram stain, which identifies them based on the chemical and physical properties of their cell walls, as gram positive or gram negative. • Gram positive bacteria have a thick cell wall, which consists of up to around 30 layers of peptidoglycan. This cell wall surrounds a monoderm, which is a single plasma membrane. Gram negative bacteria have a MUCH thinner cell wall, consisting of a single layer of peptidoglycan. This layer of peptidoglycan is sandwiched between two lipid bilayer membranes called diderms. • Gram positive and gram-negative bacteria can be differentiated by dying them with crystal violet and then washing them with a decolorizing solution. Then, a counterstain is added, for example safranin or fuchsine. Gram positive bacteria will retain the crystal violet dye and remain purple, while the gram-negative bacteria will be stained pink. • Gram positive bacteria also pick up the pink colour of the counterstain, however this is not visible when they are dyed with the darker purple colour of the crystal violet stain. • The reason for these staining differences is due to differences in cell wall structure, which is the chief difference between gram positive and gram-negative bacteria. The gram stain detects peptidoglycan, and since Gram positive bacteria have a thick, multilayered peptidoglycan layer, they retain the crystal violet dye. Gram-negative bacteria do not retain the dye for two reasons – they have an outer membrane getting in the way of the crystal violet and they lack peptidoglycan to retain the stain. • Gaining knowledge always doesn’t require expenses. So it is absolutely free. You can easily go through the topics again and again till you are through with the concept. • Thus this channel is for learners of Life science. All the lectures are very well explained. • Thank you for watching the video and subscribe to get the upcoming knowledgeable videos. • Hope you all will support in future too. • Do LIKE, SHARE AND SUBSCRIBE. • For • CELL BIOLOGY INTRODUCTION TO CELL BIOLOGY LIFE SCIENCE •    • CELL BIOLOGY  INTRODUCTION TO CELL  B...   • MARK- RECAPTURE TECHNIQUE | POPULATION ECOLOGY ||CSIR UGC NET    • MARK- RECAPTURE TECHNIQUE | POPULATIO...   • MARK- RECAPTURE TECHNIQUE •    • MARK  RECAPTURE TECHNIQUE   • #bacteria #biologyclass12 #peptidoglycan • GRAM,BACTERIA,PROKARYOTE,PEPTIDOGLYCAN,CRYSTAL VIOLET,SAFRANIN,GRAM STAINING,GRAM POSITIVE AND GRAM NEGATIVE BACTERIA,GRAM POSITIVE BACTERIA,GRAM NEGATIVE BACTERIA,MICROBIOLOGY LECTURE,MICROBIOLOGY STAINING,gram stain,gram,staining,bacteria,prokaryote,peptidoglycan,crystal violet,safranin,gram +ve,gram -ve,microbiology,anatomy,gram positive vs gram negative bacteria,difference between gram positive and gram negative bacteria,gram negative and gram positive bacteria • • ABC MODEL FOR FLOWER DEVELOPMENT/ CSIR UGC NET QUESTIONS •    • ABC MODEL FOR FLOWER DEVELOPMENT CSIR...   • HEMATOPOIESIS: LECTURE 1: IMMUNOLOGY •    • HEMATOPOIESIS: LECTURE 1 IMMUNOLOGY   • IMMUNE SYSTEM-INNATE AND ADAPTIVE IMMUNITY: LECTURE 2 IMMUNOLOGY •    • IMMUNE SYSTEM INNATE   ADAPTIVE IMMUN...   • CELLS OF IMMUNE SYSTEM: LECTURE 3 IMMUNOLOGY •    • CELLS OF IMMUNE SYSTEM : LECTURE 3: I...   • COMPLEMENT SYSTEM •    • COMPLEMENT SYSTEM CLASSICAL, ALTERNAT...   • HEMATOPOIESIS IN HINDI •    • HEMATOPOIESIS IN HINDI   • Find me on different sites: • Facebook page-   / growwithbiology   • LinkedIn -  / grow-with-0945bb1a7   • SlideShare- www.slideshare.net/GROWWITH • Youtube-    / @growwithbiology   • Instagram-  / arpita.mukherjeephd   • GRAM,STAINING,BACTERIA,PROKARYOTE,BIOLOGY,SIMPLE,PEPTIDOGLYCAN,CRYSTAL VIOLET,SAFRANIN,GRAM STAINING,GRAM POSITIVE AND GRAM NEGATIVE BACTERIA,GRAM POSITIVE BACTERIA,GRAM NEGATIVE BACTERIA,MICROBIOLOGY LECTURE,MICROBIOLOGY STAINING,gram positive,gram negative,gram stain,gram,staining,bacteria,prokaryote,biology,simple,peptidoglycan,crystal violet,safranin,gram +ve,gram -ve,microbiology,anatomy,physiology,medicine,nursing,prokaryotes,science,cells • #Positive #Negetive #Bacteria

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