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What is Blood Agar Media Complete amp Clear Overview
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❇️ Join this channel to get access to perks: • / @aladdincreations Hello Viewers !!! • My Name Is Kavindu Lakmal , Medical Laboratory Science Student From University Of Peradeniya. • I designed this video from my Text books notes internet resources. • I hope this video will helpful for your studies ... • Don't Forget to subscribe me ... • You can update with more new videos .... • Please Comment your ideas about Video .... • ***************************** HELP ME !!! ********************** • I Love to make this like videos Continuously …. • But everything need some money , So your little donation I really appreciate . • Your just 1$ donation help to design many more videos … • Become a Patron - https://www.patreon.com/user?u=4259366 • ******************************************************************** • Not Only That , Need To Design this Like Video For You Or Any Video Editing Help ??? • Contact me ... • [email protected] • Skype Name - kavindu.lakmal11 • • Thank You • • ************************************************************* • Video Script :- • Blood Agar Media • Blood agar is an enriched media , which capable of growing a range of microorganisms of clinical significance. • Fastidious organisms, such as streptococci, do not grow well on ordinary growth media. • BUT Blood agar is a type of growth medium that encourages the growth of bacteria, such as streptococci, that otherwise wouldn’t grow. • ................................................... • Also Blood contains inhibitors , for certain bacteria such as Neisseria and Haemophilus genera and the blood agar must be heated to inactivate these inhibitors and to release essential growth factors such as x factor V factor . • But When Heating of blood agar converts it into chocolate agar and supports the growth of these bacteria. • Blood agar can be made selective for certain pathogens , by the addition of antibiotics, chemicals or dyes. Examples includes crystal violet blood agar to select Streptococcus pyogens from throat swabs, and kanamycin or neomycin blood agar , to select anaerobes from pus. • Composition Of Blood Agar • Blood agar media consist of , • Pancreatic digest of casein • Papaic digest of soy meal • 0.5% NaCl • Agar • Distilled water • 5% Sheep Blood • Combine of the ingredients and final pH should be 7.3 • .................................................. • Uses Of Blood Agar Media • There 3 main uses of Blood agar , they are • Isolation organisms • Identification • Detect antimicrobial susceptibility. • After spreading specimen on blood agar media , can be examine isolated pathogen organisms colonies on blood agar media. So blood agar really important to Isolate medically significant organisms. • Also blood agar media gives good colony appearance , hemolysis patterns such as Alpha , Beta gama pigment production. • Quality control of Blood Agar • Using Quality control of blood agar , we can identify whether our media in good quality or not , If not we have to discard all media • There several method to Quality control Blood agar , • The pH of the blood agar range should from 7.2 to 7.6 at room temperature. • Incubate the plates in a carbon dioxide enriched atmosphere at 35-37°C overnight check there any contamination present or not • Check the growth of positive control organisms see colony , morphology , • In this case these positive control organisms must grow on blood agar media gives identical colony morphology , So • Staphylococcus aureus - should give good growth with white coloured colonies • Streptococcus pyogenes - should give good growth with pale colonies and beta haemolysis • And Streptococcus pneumonia - should give good growth with gray coloured colonies alpha hemolysis
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