The Discovery of Acoustic Focusing amp the Attune® Flow Cytometer

>> YOUR LINK HERE: ___ http://youtube.com/watch?v=b2ilHEnugE0

Watch more videos, learn more or request a demo at https://www.thermofisher.com/us/en/ho... • This mini-webinar discusses how the discovery of sound measurement lead to a series of discoveries culminating in the knowledge needed to use sound in particle focusing. The Attune® Acoustic Focusing Flow Cytometer is the first cytometer that uses ultrasonic waves (over 2 MHz, similar to those used in medical imaging), rather than hydrodynamic forces, to position cells into a single, focused line along the central axis of a capillary. • Excerpt from video: Where does innovation come from? • What sparks the light in an inventor's mind that initiates the sequence of thoughts leading to the grand crescendo of a great discovery? • Some would say there are no new ideas... • just tweaks and edits to existing ideas, concepts and technologies that can lead to something that will change the world around that idea forever. • Today we are going to dive in and dissect a discovery in the world of Flow Cytometry- How the use of acoustical forces to count and study particles came to be. The use of forces to focus, count and study cells and particles. • As you might guess, it's not a simple story. Instead it 's a journey through the history of studying sound waves starting with a device created back in 1866 to measure the speed of sound. This experiment lead to a series of discoveries that culminated in the invention of the world's first acoustic focusing cytometer, the Attune. • But why would you use sound instead of conventional methods? Hang tight, We'll get to that because the answer lies in the history....(watch video to get the whole story)... • A few years later as a PhD level scientist he was brought into the Los Alamos National Laboratories to work on other things that were of interest to the US Government. While he was there, he came across Gary Sultzman, a very well known cytometrist. Gary asked Greg if he would like to try to apply this to particle focusing in flow cytometry. Greg said I would be happy to... but what's flow cytometry? • Well, Conventional flow cytometry uses hydrodynamic focusing to move and maintain particles or cells in the center of the interrogating laser. This system uses a rapidly moving sheath fluid to pull particles or cells into a central core and then to pass them in front of a laser that can then read scatter and fluorescence signals from the particles or cells. The problem with this system is that, as more sample is added, the core increases in diameter and the cells can now pass through regions of the laser spot that are not as intense as the center. Moreover, this limits the amount of sample that can be passed to a maximum of about 150 microliters per minute. • Not bad, but not great. It was time to innovate. • The difference with acoustic focusing is that to a great extent you de-couple focusing from the lateral movement of cells. In this way, particles or cells are focused in the acoustic device prior to entering into the manifold where they are met with sheath fluid. With acoustic focusing, sample rates can be increased by over 10 times those in hydrodynamic focusing instruments while maintaining focus. • So, what was originally a bootleg project ultimately became an official project in Greg Kaduchak's lab. Here he was joined in his efforts by two scientists, Mike Ward and Greg Goddard. Together they worked to advance the technique of making stable capillaries that are the key to using acoustics to focus particles, cells and bacteria, into the center of a capillary. • In 2005, Kaduchak and Ward started their own company in Los Alamos in order to build the world's first acoustic focusing flow cytometer. • In early 2008, Life Technologies, then Invitrogen, a company known not only for developing new technologies but also for scouting out the best innovations went to Los Alamos to review the technology. Life Technologies saw something special and knew they could help provide the resources to get this out to researchers quickly. • Life Technologies brought Greg and Mike's small company to the team and moved the technology and the scientists into the Molecular Probes site in Eugene, Oregon. 17 months later, this dynamic team of flow experts and Molecular Probes scientists delivered the Attune acoustic focusing cytometer based on this principle of particle movement using acoustics. It was the world's first instrument created using this technology, built open a series of sound related discoveries. • And now you know how an experiment on a boat in the early 1800's triggered a series of events that ultimately opened doors in Flow Cytometry research. Now that this innovation has made it to the lab, what new innovations will this lead to in your world?

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