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MeselsonStahl Experiment
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Meselson and stahl experiment | Class 12 Biology • About This Video ........................ • In This Video Lecture You Will Learn Basic Concept Of Meselson And Stahl Experiment • The three hypothesis of DNA replication were evaluated by Mathew Meselson and • centrifugal forces generated by the ultracentrifuge caused the cesium ions to migrate • They dissolved the DNA in cesium chloride and then spun it at a very high speed in • toward the bottom of the centrifuge tube, creating a gradient of CsCl, and thus of • a medium containing heavy isotope of nitrogen, 15N, which became incorporated into • an ultra-centrifuge. DNA strands of different densities got separated. The enormous • density. Each DNA floats or sinks in the gradient until it reaches the position where its • to the N14 medium and collected the DNA at various intervals. • the bases of the bacterial DNA. After several generations, the DNA of these bacteria • than N14 strands, they migrate farther down the tubes to a denser region of the cesium • was denser than that of bacteria grown in a medium containing the lighter isotope of • nitrogen, N14. Meselson and Stahl then transferred the bacteria from the N15 medium • Franklin Stahl of the California Institute of Technology in 1958. They grew bacteria in • density exactly matches the density of cesium there. Because N15 strands are denser • chloride gradient. • The DNA collected immediately after the transfer, was all dense. However, after the • After the second round of replication, two density classes of DNA were observed one • Meselson and Stahl interpreted their results as follows: after the first round of replication. Thus, this experiment clearly confirmed the prediction of the Watson-Crick • bacteria completed their first round of DNA replication in the N 14 medium, the density • of their DNA had decreased to a value intermediate between N14- DNA and N15 - DNA. • molecule and one light strand. When this hybrid duplex replicated, it contributed one • intermediate and one equal to that of N14 - DNA. • each daughter DNA duplex was a hybrid possessing one of the heavy strands of parent • heavy strand to form another hybrid duplex and one light strand to form a light duplex • model that DNA replicates in a semi-conservative manner. • About This Channel..................... • I make these videos cause I love to draw and connect the complexity of science and medicine into art. I'm not saying. I'm 100% correct in all my videos, but I do try to obtain the information from credible sources. • One-stop destination for all biology lectures. • _ students in class 11, 12 or appearing for competitive medical exams ( NMDCAT ) will find it very beneficial • _ The videos are categories under various heading to make the students life simplified • Visit My Other Channel Playlist ..................... • NMDCAT PART 1: • New 1st Year Biology • NMDCAT PART 2: • New 2nd Year Biology • Class 11th Lectures: • New 1st Year Biology • Class 12th Lectures: • New 2nd Year Biology • Class 10th Lectures: • • Matric Biology • Subscribe My Channel: • / aliacademya7r • Social Link........................ • Instagram: • https://instagram.com/ranaalibiologis... TWINS
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