Reverse Transcriptase Reaction in 10 minutes – Enzyme Processivity

>> YOUR LINK HERE: ___ http://youtube.com/watch?v=gXE-9T9Mj4c

If you have questions or comments please go to the new channel Ciencia y Science and ask your questions there so that I can answer them. / @cienciayscience • The cDNA synthesis tutorial video is the follow up step after isolating RNA. We are able to synthesize cDNA from RNA through a process called reverse transcription (RT). RT is a process that does not occur in mammalian cells and it is not part of the Central Dogma in Biology. In order to perform RT we make use of a retrovirus enzyme called reverse transcriptase, which is able to synthesize cDNA from RNA. • [Time stamps] • 0:00 - introduction • 3:43 - components • 5:17 - measuring RNA concentration • 6:26 - loading samples onto Take3 Trio plate • 8:27 - BioTek Cytation5 software • 12:00 - Calculations to run cDNA synthesis • 16:33 - Mixing all components for RT • 28:22 - Running RT using thermocycler • Links to other tutorial videos associated with this video • 1) Isolating RNA from primary cells • • Isolating RNA from primary cells • 2) Running qPCR of cDNA • • Running qPCR of cDNA

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