AFB staining procedure acid fast stain zn stain for mycobacterium tuberculosis











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About video • AfB STAIN OR ACID FAST STAIN OR ZN STAIN • • PROCEDURE • Air dry and heat fix a thin film of microorganisms. Allow the slide to cool. • 2. Flood the slide with Carbol fuchsin. Steam the slide with a Bunsen burner over the sink. Let the slide set for 5-10 minutes. Rinse with water. • 3. Flood slide with Acid Alcohol for 30 seconds. Rinse with water. • 4. Counterstain by flooding the slide with Methylene Blue for 1-2 minutes . Rinse with water. • 5. Dry the slide by putting it between the pages of a book of Bibulous paper. • 6. View organisms using the oil immersion objective of your microscope. • #afbstaingprocedure • #znstain • #afbstain • instagram.com/manojcricket15gmail.com8529 ( instagram link )

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