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Potato Dextrose agar PDA media culture media lecture 29
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this is the video lecture regarded to... • • in this video lecture we will explain.... • in this video lecture we will explain the following content related to this lecture • 1. introduction • PDA is a general purpose or basal growth media for fungi. • Chloramphenicol, Tartaric Acid and Chlortetracycline can be added as selective agents to inhibit bacterial growth • Recommended by APHA and FDA for plate counts of yeasts and molds in the examination of foods and dairy products. • 2. composition • 3. Uses • Used for the detection of yeasts and molds in dairy products, prepared foods and also from clinical specimens. • PDA with Chloramphenicol is recommended for the selective cultivation of fungi from mixed samples. • PDA with Chlortetracycline is recommended for the microbial enumeration of yeast and mold from cosmetics. • It also aids in cultivating and differentiating pathogenic and non-pathogenic fungi. • PDA is also useful for maintaining stock cultures of certain dermatophytes. • 4. Principle • • Dextrose act as a carbohydrate source which serves as a growth stimulant • Potato infusion provides a nutrient base for the luxuriant growth of most fungi. • Agar is added as the solidifying agent. • The addition of supplement like chloramphenicol (25 mg), Chlortetracycline (40 mg) and tartaric acid (1.4 gm) inhibit bacterial growth. • Distill water mix all the ingredients. • 5. Preparation • Suspend 39 grams in 1000 ml distilled water. • Heat to boiling to dissolve the medium completely. • Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. • Cool the medium to approximately 50°C and pour into sterile Petri dishes. • Inoculate and streak the medium with one isolated pure colony. • Incubate in an aerobic atmosphere at 35ºC for 24-48 hours. • Observe the growth of fungus. • 7. Results • lecture on all culture media used in microbiology • • Lecture or playlist of all culture me... • all biochemical test video lectures • • All biochemical test video lecture • Different screening technique in recombinant DNA technology • • Different screening Techniques in rDN... • DNA replication in prokaryotes • • DNA replication in prokaryotes • Lectures of vectors in recombinant DNA technology • • Lectures of different vectors in rDNA... • all hematology video lectures • • Hematology test lectures • all lecture on DNA as genetic material • • extra video lectures • All MHC lectures • • Over view of MHC molecules: MHC lectu... • all virology video lectures • • virology lectures • lectures on physical barriers of innate immune system • • Lectures of physical barriers of inna... • lectures on antibiotics • • antibiotic vedio lectures • Lectures on cell biology • • cell biology lectures • lectures on antiviral drugs • • antiviral drugs • lectures on antifungal • • Anti fungal video lectures • lectures on celiac disease • • celiac disease videos lectures • lectures on life cycle of basic viruses • • life cycle of basic viruses • lectures on chikungunia disease • • Lectures or playlist of chikungunya d... • lectures on DNA replication in prokaryotes and eukaryotes • • DNA replication in prokaryotes and eu... • lectures on recombinant DNA technology • • Lectures of Recombinant DNA Technolog... • lecture on B. Cell biology • • B cell biology lectures • lecture on T. Cell biology • • T cell biology lectures • lecture on eukaryotic translation (protein synthesis) • • Playlist or lectures of Eukaryotic tr... • lecture on prokaryotic translation (protein synthesis) • • Lectures on Translation in prokaroyotes • immunology lectures • • Immunology lectures • lectures on complement system • • Lectures or playlist of complement sy... • lectures on antibodies • • Antibody lectures • lectures on inflammation • • Inflammation lectures • lectures on hypersensitivity • • Hypersensitive lectures • all cytokine lectures • • Lectures on Cytokine
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