CHT™ Ceramic Hydroxyapatite Syringe Slurry Transfer and Packing in a ProcessScale Column

>> YOUR LINK HERE: ___ http://youtube.com/watch?v=jhgvHq52DP8

For more information, http://bio-rad.com/processapplications • This video demonstrates guidelines for packing process-scale columns with CHT media by syringe slurry transfer. Many types of columns with motorized pistons can introduce the chromatography media without a pump, by using the top adapter in a syringe-style to draw the slurry into the column via a suction. This is typically called the syringe method. These guidelines for handling and packing are recommended for all CHT users to ensure reproducible and high-quality results. Please download the CHT Application Guide at http://bio-rad.com/chtguide for specific details on recommended buffers and slurry calculations. • This innovative mixed-mode media offers unique separation properties and unparalleled selectivity and resolution for a variety of molecules. These media can be used at any stage in a process from initial capture to final polishing. Two types of CHT are available in three particle sizes (20, 40, and 80 µm): • • Type I has a higher protein binding capacity and better capacity for acidic proteins • • Type II has a lower protein binding capacity but offers better resolution of nucleic acids and certain proteins; it also has a very low affinity for albumin and is particularly well-suited for the purification of many species and classes of immunoglobulins • Bio-Rad's Process Chromatography group offers a full range of chromatography solutions for biomolecule purifications. Visit http://bio-rad.com/process to see our full range of process chromatography media. • We Are Bio-Rad. • Our mission: To provide useful, high-quality products and services that advance scientific discovery and improve healthcare. At Bio-Rad, we are united behind this effort. These two objectives are the driving force behind every decision we make, from developing innovative ideas to building global solutions that help solve our customers' greatest challenges. • Connect with Bio-Rad Online: • Website: http://www.bio-rad.com/ • LinkedIn: / 1613226 • Facebook: / biorad • Twitter: / bioradlifesci • Instagram: @BioRadLabs • Snapchat: @BioRadLabs

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