Nearest Neighbor Method

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A general method for calculating the melting temperature of DNA • Table of Contents: • 01:53 - Fundamental Thermodynamic Relationships • 02:40 - The Nearest Neighbor Approach • 04:59 - Calclulating duplex thermodynamics • 11:52 - A sample calculation • 15:19 - Deriving the equation for Tm • 20:33 - How the parameters were determined

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