Quantitative RTPCR of Nanofluidic Samples Protocol Preview

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Watch the Full Video at https://www.jove.com/v/61132/high-thr.... • High-Throughput Quantitative RT-PCR in Single and Bulk C. elegans Samples Using Nanofluidic Technology - a 2 minute Preview of the Experimental Protocol • Laetitia Chauve, Jérémie Le Pen, Francesca Hodge, Pia Todtenhaupt, Laura Biggins, Eric A. Miska, Simon Andrews, Olivia Casanueva • Babraham Institute,; University of Cambridge, Gurdon Institute; University of Cambridge, Department of Genetics; Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus; The Rockefeller University, Laboratory of Virology and Infectious Disease; • In this article a high-throughput protocol for fast and reliable determination of gene expression levels in single or bulk C. elegans samples is described. This protocol does not require RNA isolation and produces cDNA directly from samples. It can be used together with high-throughput multiplexed nanofluidic real-time qPCR platforms. • Visit https://www.jove.com?utm_source=youtube utm_medium=social_global utm_campaign=reseach-videos-2022 to explore our entire library of 14,000+ videos of laboratory methods and science concepts. • JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. • Subscribe to our channel: / jovejournalofvisualizedexperiments

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