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Powering Neurons with Light Optogenetics and the Maestro multielectrode array MEA system
>> YOUR LINK HERE: ___ http://youtube.com/watch?v=koTQG-LJ6gg
Using the Lumos optical stimulator and Axion's multiwell Maestro MEA system to study neural network activity with optogenetics. • Learn more: https://www.axionbiosystems.com/appli... • Neurons on the left side of the dish have been transduced with a blue light-activated ion channel (channel rhodopsin, ChR2), the neurons on the right have not. Watch as blue light induces activity in the ChR2-labeled left-side neural population, that then spreads to, and activates the unlabeled right-side neural population. • • What is a multiwell MEA assay? • Axion’s microelectrode array (MEA), also known as multielectrode array, plates have a grid of tightly spaced electrodes embedded in the culture surface of each well. Electrically active cells, such as neurons, can be cultured over the electrodes. Over time, as the cultures become established, they form cohesive networks and present an electrophysiological profile. The resulting electrical activity, spontaneous or induced firing of neurons, is captured from each electrode on a microsecond timescale providing both temporally and spatially precise data. • What is optogenetics? Using light to control cells. • Optogenetics is a technique that involves the use of light to control cell function. Cells are first genetically modified to express light-sensitive ion channels, called opsins. Then, light can be used to activate the opsin. The most well-known opsins are light-gated ion channels that can control the excitability of the cell membrane. When activated by the opsin-specific wavelength of light, the channels open allowing ions to flow across the cell membrane to either excite or inhibit the cell. Optogenetics enables precise control over a targeted cell population.
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