CryoSPARC Live Walkthrough

>> YOUR LINK HERE: ___ http://youtube.com/watch?v=lrIHBb7Dr8w

Watch the cryoSPARC v3.0 cryoSPARC Live Launch Video: • Introducing cryoSPARC v3.0 the new ... • Join us while we process cryo-EM data (EMPIAR-10288) using cryoSPARC Live, an all-new cryo-EM single particle analysis software system built by Structura Biotechnology. We'll walk you through the entire interface, and process this dataset from start to finish. • CryoSPARC Live is available with the cryoSPARC software platform, which you can get here: https://cryosparc.com/ • For more information about cryoSPARC Live, visit our guide, which has in-depth tutorials and documentation: https://guide.cryosparc.com/live/abou... • Download EMPIAR-10288, the dataset used in this walkthrough: https://www.ebi.ac.uk/pdbe/emdb/empia... • Note: The gain reference file provided in this dataset is in a file format (.dm4) that cryoSPARC doesn't support. Download a compatible version of the gain reference to use in cryoSPARC here: • https://structura-assets.s3.amazonaws... • Website: https://structura.bio/ • Twitter: / structurabio • Parameters Used For Data Processing: • --------------------------------- • Microscope/Camera Parameter • Raw pixel size (Å): 0.86 • Accelerating voltage (kV): 300 • Spherical aberration (mm): 2.7 • Total exposure dose (e/Å^2): 58 • Advanced: • Flip gain ref in Y? : True • Blob Picker • Minimum particle diameter (Å): 100 • Maximum particle diameter (Å): 150 • Advanced: • Use elliptical blob: True • Particle Extraction • Extraction box size (pix): 320 • Advanced: • Fourier crop to box size (pix): 240

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